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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is implementing whole-genome sequencing (WGS) for enhanced surveillance of Listeria monocytogenes isolates associated with foodborne outbreaks. WGS provides several advantages over traditional subtyping methods like pulsed-field gel electrophoresis (PFGE). Which of the following is the MOST significant advantage of WGS in this context?

A) Higher discriminatory power for resolving phylogenetic relationships and identifying outbreakclusters.
B) Easier standardization and inter-laboratory comparison of data.
C) Lower cost per isolate compared to PFGE.
D) Faster turnaround time for obtaining results.

2. During the investigation of a foodborne illness outbreak linked to raw sprouts, several bacterial isolates are obtained from patient stool samples and the implicated food source. Which of the following molecular methods would be MOST useful for establishing a definitive link between the clinical isolates and the food isolate?

A) Pulsed-field gel electrophoresis (PFGE)
B) Gram staining and biochemical profiling
C) Serotyping based on O and H antigens
D) Antimicrobial susceptibility testing

3. A patient develops severe, watery diarrhea shortly after starting a course of clindamycin. Stool testing using a cytotoxicity assay is positive. This result is most consistent with infection by which organism?

A) Campylobacter jejuni
B) Clostridioides difficile
C) Norovirus
D) Giardia lamblia

4. A patient presents with perianal itching, particularly at night. Microscopic examination of cellophane tape preparation from the perianal area reveals oval-shaped eggs with one flattened side. The MOST likely causative organism is:

A) Enterobius vermicularis
B) Strongyloides stercoralis
C) Ascaris lumbricoides
D) Trichuris trichiura

5. A patient with a history of recurrent Clostridioides difficile infection (CDI) is being considered for fecal microbiota transplantation (FMT). Pre-FMT screening of the donor stool sample should include comprehensive testing to exclude potential transmission of enteric pathogens. Which of the following would be the MOST critical to rule out in the donor stool?

A) Detection of common antibiotic resistance genes not currently expressed.
B) Evidence of asymptomatic carriage of multidrug-resistant organisms (MDROs) such as vancomycin- resistant enterococci (VRE) or carbapenem-resistant Enterobacterales (CRE).
C) Presence of non-toxigenic C. difficile strains.
D) Quantification of the overall microbial diversity using 16S rRNA gene sequencing.

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